On Investigation of Irradiation Effect on Biosensor Counterparts Interaction Based on Advanced Spectrophotometric Studies

Abstract

Due to their ease of use, spectrophotometric studies are traditionally applied for the qualitative analysis of solutions and the effects of external influences. In brief, the analysis of spectrographs is based on the analysis of maximum (minimum) absorption values and the corresponding wavelengths. All other (non-extremal) absorption values are eliminated from the consideration, which in turn involves the risk of losing significant information, e.g., on protein conformational changes caused by external factors. Such effects are of importance for bovine serum albumin, which plays the role of a stabiliser for the biosensor receptor layer. In the given work, we develop an advanced numerical method, taking into account the entire range of spectrum wavelengths, which enables us to study more complicated effects and relations within the biochemical system. For the purpose of proving the effectiveness of the method, we study here the effect of ultraviolet radiation on biosensor components with the help of ultraviolet–visible spectroscopy. The primary processing of the spectra was carried out on the basis of absorption-extrema analysis. Further, we apply the dimension reduction technique in order to isolate the wavelengths of greatest importance in the process of the conformation of the studied protein and its complexes. The conducted studies allowed us to prove the accelerated conformational changes in the protein and its complexes under the influence of ultraviolet radiation.